Xyloglucan Galactosyl- and Fucosyltransferase Activities from Pea Epicotyl Microsomes
نویسندگان
چکیده
منابع مشابه
Xyloglucan galactosyl- and fucosyltransferase activities from pea epicotyl microsomes.
Microsomal membranes from growing tissue of pea (Pisum sativum L.) epicotyls were incubated with the substrate UDP-[14C]galactose (Gal) with or without tamarind seed xyloglucan (XG) as a potential galactosyl acceptor. Added tamarind seed XG enhanced incorporation of [14C]Gal into high-molecular-weight products (eluted from columns of Sepharose CL-6B in the void volume) that were trichloroacetic...
متن کاملIdentification of EDTA-Soluble Polysaccharides from Pea Epicotyl Cell Walls and Their Interaction with Xyloglucan
Nascent pectin and glucuronoxylan were prepared from membrane-bound enzymes obtained from pea epicotyls. They had previously been shown to exhibit a proteinand pH-dependent pattern of binding to cell wall ghosts and to xyloglucan extracted from cell walls prepared from pea epicotyls; maximum binding required a pH of 3-4, and the presence of cell wall proteins, namely assemblins. To determine wh...
متن کاملXyloglucan fucosyltransferase, an enzyme involved in plant cell wall biosynthesis.
Cell walls are crucial for development, signal transduction, and disease resistance in plants. Cell walls are made of cellulose, hemicelluloses, and pectins. Xyloglucan (XG), the principal load-bearing hemicellulose of dicotyledonous plants, has a terminal fucosyl residue. A 60-kilodalton fucosyltransferase (FTase) that adds this residue was purified from pea epicotyls. Peptide sequence informa...
متن کاملBiochemical characterization and molecular cloning of an alpha-1,2-fucosyltransferase that catalyzes the last step of cell wall xyloglucan biosynthesis in pea.
Pea microsomes contain an alpha-fucosyltransferase that incorporates fucose from GDP-fucose into xyloglucan, adding it preferentially to the 2-O-position of the galactosyl residue closest to the reducing end of the repeating subunit. This enzyme was solubilized with detergent and purified by affinity chromatography on GDP-hexanolamine-agarose followed by gel filtration. By utilizing peptide seq...
متن کاملIn vitro activities of four xyloglucan endotransglycosylases from Arabidopsis.
Xyloglucan endotransglycosylases (XETs) are encoded by a gene family in Arabidopsis thaliana. These enzymes modify a major structural component of the plant cell wall, xyloglucan, and therefore may influence plant growth and development. We have produced four Arabidopsis XETs (TCH4, Meri-5, EXGT and XTR9) using the baculovirus/insect cell system and compared their biochemical activities. TCH4, ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1997
ISSN: 1532-2548,0032-0889
DOI: 10.1104/pp.114.1.245